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Combining electrochemical impedance spectroscopy and surface plasmon resonance into one simultaneous read-out system for the detection of surface interactions

 
: Vandenryt, T.; Pohl, A.; Grinsven, B. van; Thoelen, R.; Ceuninck, W. de; Wagner, P.; Opitz, J.

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Postprint (PDF; )

Sensors. Online journal 13 (2013), No.11, pp.14650-14661
http://www.mdpi.com/journal/sensors
ISSN: 1424-8220
English
Journal Article, Electronic Publication
Fraunhofer IZFP, Institutsteil Dresden ( IKTS-MD) ()

Abstract
In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1x PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS.

: http://publica.fraunhofer.de/documents/N-281995.html