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Combining phase contrast and immunofluorescence images using geometric hashing

: Becker, T.; Schultz, S.; Rapoport, D.H.; Mamlouk, A.M.


Institute of Electrical and Electronics Engineers -IEEE-; IEEE Signal Processing Society; IEEE Engineering in Medicine and Biology Society -EMBS-:
IEEE 10th International Symposium on Biomedical Imaging, ISBI 2013. From Nano to Macro. Vol.2 : San Francisco, California, USA, 7 - 11 April 2013
New York, NY: IEEE, 2013
ISBN: 978-1-4673-6456-0 (Print)
ISBN: 978-1-4673-6454-6
ISBN: 978-1-4673-6455-3
International Symposium on Biomedical Imaging (ISBI) <10, 2013, San Francisco/Calif.>
Conference Paper
Fraunhofer EMB ()

The analysis of in vitro cultured stem cells is a challenging task. One particularly important question pertains the relationship between proliferation and differentiation. Here, we introduce an image-based method to combine information about cellular genealogies with an analysis of the protein expression of cells in a culture dish. The method uses a time-series of microscopical images and a immunofluorescence (IF) image. The time-series can be used to obtain the genealogical nexus, while the IF-image displays the expression of a chosen marker-protein within each cell. The task was then to find an algorithm, which automatically maps the last image of the time-series onto the immunofluorescence image. Our solution to this problem is to use a cell detection algorithm in the time-lapse images and a cell nuclei detection in the IF-images to determine the position of the cells. Then, the cell positions are used to match the images with a geometric hashing based method. The robustness of the implemented algorithm is demonstrated using microscopical reference data. In addition, the algorithm was used to estimate the displacement of the cell nuclei (IF image) relatively to the cell shape position, i.e. the centroids in the time-lapse data.