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Exact box-car lifetime measurement of dye fluorescence

: Danz, N.


Sensors and Actuators. A 116 (2004), No.2, pp.312-319
ISSN: 0924-4247
Journal Article
Fraunhofer IOF ()
fluorescence lifetime; box-car integration; pulse-lifetime measurement; photobleaching; rhodamine

The box-car or pulse-lifetime method is widely used for fluorescence lifetime measurement. The experimentally determined dye molecule fluorescence lifetime is demonstrated to be affected by bleaching effects. A modified experimental scheme is introduced to enable for the separation of excitation-induced bleaching and effects of the undisturbed dye molecules. A theoretical description of this measurement scheme is applied to experimental fluorescence time traces of rhodamine 6 G-doped PMMA layers to determine lifetime independently of the integration time with an accuracy of 1%.