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Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G

 
: Schenk, J.A.; Fettke, J.; Lenz, C.; Albers, K.; Mallwitz, F.; Gajovic-Eichelmann, N.; Ehrentreich-Förster, E.; Kusch, E.; Sellrie, F.

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Journal of biotechnology 158 (2012), No.1-2, pp.34-35
ISSN: 0168-1656
ISSN: 1389-0352
ISSN: 1873-4863
English
Journal Article
Fraunhofer IBMT ()

Abstract
The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14. kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). This SLPI contamination lead to a failure of the fiber-optic based competitive fluorescence assay to detect progesterone in milk. Purification of the monoclonal antibody using protein A columns circumvented this problem.

: http://publica.fraunhofer.de/documents/N-206751.html