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A novel approach to measure optical density in a hand-held pipette

 
: Patel, Mitul; Mankodiya, Kunal; Schöning, Sebastian; Opp, Alexander; Domnich, Alexej; Hofmann, Ulrich G.

Deutsche Gesellschaft für Biomedizinische Technik -DGBMT-:
BMT 2010, 44. DGBMT Jahrestagung. CD-ROM : Proceedings; 3-Länder-Tagung D-A-CH, Rostock, 05. - 08.Oktober 2010, Kongresszentrum Hohe Düne Rostock-Warnemünde
Berlin: De Gruyter, 2010 (Biomedizinische Technik 55.2010, Supplement 1)
pp.36-39
Deutsche Gesellschaft für Biomedizinische Technik (Jahrestagung) <44, 2010, Rostock>
German
Conference Paper, Journal Article
Fraunhofer IPA ()
LED; optical measurement; optische Meßtechnik; Pipettieren; Lichtsensor

Abstract
This paper presents the design and fabrication of a miniaturized light detection system for optical density measurements based on intelligent opto-electronic sensor technology. The design allows the implementation of light absorption and measurement schemes and can be potentially integrated as a modular approach in a hand-held pipette. The electronic circuit for optical density measurement was fabricated to incorporate a high intensity light emitting diode (LED) as a light source and an opto-electronic light intensity sensor, both controlled by a microcontroller. Optical density measurements were demonstrated using Coomassie Blue R250 dye in a flow-cell cuvette and in commercial pipette tips. We developed a test-rig that proves the feasibility of our approach to conduct optical density measurements in a hand-held pipette and verified our results with a commercial spectrophotometer.

: http://publica.fraunhofer.de/documents/N-203438.html