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Surfactant protein D modulates allergen particle uptake and inflammatory response in a human epithelial airway model

 
: Schleh, Carsten; Rothen-Rutishauser, Barbara M.; Blank, Fabian; Lauenstein, Hans D.; Nassimi, Matthias; Krug, Norbert; Braun, Armin; Erpenbeck, Veit; Gehr, Peter; Hohlfeld, Jens M.

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Postprint (1.6 MByte; PDF; )

Respiratory research. Online journal 13 (2012), Art. 8, 34 pp.
http://respiratory-research.com/
ISSN: 1465-993X
ISSN: 1465-9921
English
Journal Article, Electronic Publication
Fraunhofer ITEM ()
optical imaging; T-cell proliferation; allergen; dendritic cell; macrophage

Abstract
Pollen starch granules (PSGs) are allergen particles that get into contact with pulmonary surfactant and phagocytes in the terminal airways. In this study, the effects of surfactant protein D (SP-D) on the interaction of PSGs with phagocytes and on the pulmonary clearance of PSGs were determined. Fluorescently labeled PSGs were incubated in vitro with murine lung macrophages or dendritic cells (DCs) ± recombinant rat SP-D (rrSP-D). In addition, the effect of SP-D on uptake of PSGs by lung macrophages and DCs was studied in vivo. Furthermore, PSGs were instilled in Balb/c mice and the effects of SP-D on total lung clearance were assessed by optical imaging. SP-D treatment increased the number of PSG-positive macrophages and DCs in vitro. Furthermore, SP-D accelerated uptake/binding by alveolar macrophages and reduced the number of PSG-positive tissue macrophages and DCs at 24 hours. However, SP-D did not affect total lung clearance of PSGs and it did not enhance the T-cell proliferation induced by PSG-positive DCs. In conclusion, SP-D increased PSG-positive cells in vitro and accelerated PSG binding/uptake in vivo. The observed effects were limited to cellular clearance mechanisms and did not affect the total clearance of PSGs from the lung.

: http://publica.fraunhofer.de/documents/N-195496.html