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Revised guides for organ sampling and trimming in rats and mice - Part 1

A joint publication of the RITA and NACAD groups
: Ruehl-Fehlert, C.; Kittel, B.; Morawietz, G.; Deslex, P.; Keenan, C.; Mahrt, C.R.; Nolte, T.; Robinson, M.; Stuart, B.P.; Deschl, U.

Experimental and Toxicologic Pathology 55 (2003), No.2-3, pp.91-106
ISSN: 0940-2993
Journal Article
Fraunhofer ITEM ()
guideline; mammary gland; clitoral gland; preputial gland; Zymbal's gland; tongue; salivary gland; mandibular gland; parotid gland; sublingual gland; extraorbital lacrimal gland; esophagus; stomach; intestine; gall bladder; Pancreas; rat; mouse; standardization; skin; lymph node; Esophagus; Trachea; pharynx - cancer; Larynx; liver

This is the first part of a series of three articles on trimming instructions of rat and mouse protocol organs and tissues in regulatory type toxicity studies. It is based on the experience made in the European RITA and American NACAD working groups and is an extended revision of trimming guides published in 1995 (Bahnemann et al.). The optimum localization for tissue preparation, the sample size, the direction of sectioning and the number of sections to be prepared is described organ by organ. These descriptions are illustrated for each organ by a schematic drawing and a macro-photograph showing the plane of section as well as a low power view of the H&E stained slide demonstrating the optimum "end-produc".
This revision will improve the quality and efficiency of routine procedures and facilitate daily work in the histotechnical lab. It will promote intra- and inter-study reproducibility and comparability and thus lead to a further coherence within each study and improvement of the validity of historical control data.