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Testing possibilities for establishing nanodiamond-DNA-conjugates

: Pohl, A.; Joch, S.; Michael, J.; Boschke, E.; Quenzel, P.; Schreiber, J.; Lapina, V.; Opitz, J.


Ramanujam, N. ; Optical Society of America -OSA-, Washington/D.C.:
Clinical and biomedical spectroscopy and imaging II : 24 - 26 May 2011, Munich, Germany. Part of SPIE/OSA European Conferences on Biomedical Optics
Bellingham, WA: SPIE, 2011 (SPIE Proceedings 8087)
ISBN: 978-0-8194-8684-4
ISSN: 0038-7355
ISSN: 1605-7422
Paper 80871T
Conference "Clinical and biomedical spectroscopy and imaging" <2, 2011, Munich>
European Conferences on Biomedical Optics <2011, Munich>
Conference Paper
Fraunhofer IZFP, Institutsteil Dresden ( IKTS-MD) ()

We report on the biofunctionalization of nanodiamond (ND) surfaces in a two step procedure: chemical modification, resulting in homogeneous and defined surface coating, with following addition of ssDNA. Carboxylation, thymidine coupling and amination methods for chemical modification of diamond surfaces for further functionalization experiments were applied. To enable the coupling process, biomolecules were also chemically modified with functional groups (-NH2). FTIR spectroscopy, fluorescence microscopy and gel electrophoresis were applied for characterizing modified ND particles and bioconjugates and for controlling the coupling success.