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Comprehensive characterization of two different Nicotiana tabacum cell lines leads to doubled GFP and HA protein production by media optimization

: Ullisch, David A.; Müller, Christina A.; Maibaum, Sabrina; Kirchhoff, Janina; Schiermeyer, Andreas; Schillberg, Stefan; Roberts, Jean L.; Treffenfeldt, Wiltrud; Büchs, Jochen


Journal of bioscience and bioengineering 113 (2012), No.2, pp.242-248
ISSN: 1389-1723
Journal Article
Fraunhofer IME ()
BY-2 cells; influenza hemagglutinin; media optimization; nitrogen source; oxygen transfer rate; plant cell culture; respiration activity monitoring system (RAMOS); shake flasks

For over two decades, plant cell cultures have been a promising research platform to express recombinant and therapeutic proteins such as hormones, growth factors, full-size antibodies and antigens. Chosen as a good host for manufacturing recombinant proteins, the Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cell line has been studied in shake flasks by offline analysis of only a few growth parameters. The objective of this study is to comprehensively characterize the growth of a transgenic BY-2 cell line and to investigate the expression profile of the model protein GFP. Based on the correlations between nutrient consumption, cell growth and product formation, the intention is to improve the standard MS-medium. Hereby, multiple growth parameters were analyzed offline and online by using a Respiration Activity monitoring system (RAMOS). A reproducibly observed shift of the oxygen transfer rate (OTR) could be identified to indicate ammonium depletion in the medium. Co ncurrent with this ammonium depletion, the total protein concentration began to decrease. After the MS-medium was improved, the GFP concentration nearly doubled. When this improved ammonium enriched medium was applied to another transgenic tobacco cell line similar improvements to the amount of the glycoprotein influenza hemagglutinin (HA) produced by Nicotiana tabacum NT-1 cells could be achieved. Ultimately, this combined offline and online analysis can be successfully used for further cell line characterization and media optimization to improve growth and boost target product formation.