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Improving tobacco (Nicotiana tabacum) suspension cells as a molecular farming platform: A protease knock down approach

: Mandal, M.K.

Fulltext urn:nbn:de:0011-n-1804768 (5.0 MByte PDF)
MD5 Fingerprint: 0aad172c6e6e04ea09e8eecb2190659c
Created on: 23.09.2012


Stuttgart: Fraunhofer Verlag, 2011, III, 139 pp.
Zugl.: Aachen, TH, Diss., 2011
ISBN: 3-8396-0294-7
ISBN: 978-3-8396-0294-2
Dissertation, Electronic Publication
Fraunhofer IME ()
Angewandte Forschung; applied research

The first endogenous matrix-metalloproteinase NtMMP1 from tobacco BY-2 cells was characterized in detail. Transgenic BY-2 cell lines expressing antisense-RNA against different endogenous proteases (aspartic, cysteine, matrix-metallo and serine) separately (single-antisense) or simultaneously (multi-antisense) was generated. The inhibition of endogenous protease(s) in those lines was verified and the stability of recombinant protein(s) was tested.