Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Automated clonal selection of high-producing hybridoma colonies from methylcellulose-based, semi-solid medium using the cell separation robot CellCelector

 
: Zoldan, Katharina; Knauer, Jens; Lehmann, Jörg

:
Fulltext (PDF; )

Nature methods (2010), Application Notes, Art.7719, 2 pp.
ISSN: 1548-7091
ISSN: 1548-7105
English
Journal Article, Electronic Publication
Fraunhofer IZI ()

Abstract
Monoclonal antibodies are important tools in research, diagnostics and therapy. Thus it is necessary to speed up the selection of high-producing hybridoma cell lines as it is a bottleneck in process development. Using the CellCelector™ to automatically detect and harvest in situ screened hybridoma colonies from semi-solid medium provides a rapid, simple and cost-effective alternative for faster cloning and recloning of hybridoma cell lines.

: http://publica.fraunhofer.de/documents/N-178141.html