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BAC-derived diagnostic markers for sex determination in asparagus

: Jamsari, A.; Nitz, I.; Reamon-Büttner, S.M.; Jung, C.


Theoretical and applied genetics 108 (2004), No.6, pp.1140-1146
ISSN: 0040-5752
Journal Article
Fraunhofer ITEM ()

A HindIII BAC (bacterial artificial chromosome) library of asparagus (Asparagus officinalis L.) was established from a single male plant homozygous for the male flowering gene (MM). The library represents approximately 5.5 haploid genome equivalents with an average insert size of 82 kb. A subset of the library (2.6 haploid genome equivalents) was arranged into DNA pools. Using nine sex-linked amplified fragment length polymorphism (AFLP) and two sequence-tagged site (STS) markers, 13 different BAC clones were identified from this part of the library. The BACs were arranged into a first-generation physical map around the sex locus. Four PCR-derived markers were developed from the BAC ends, one of which could be scored in a co-dominant way. Using a mapping population of 802 plants we mapped the BAC-derived markers to the same position close to the M gene as the corresponding AFLP and STS markers. The markers are useful for further chromosome walking studies and as diagnos tic markers for selecting male plants homozygous for the M gene.