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Physical map of the Azoarcus sp. strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome sequencing and functional analysis

: Battistoni, F.; Bartels, D.; Kaiser, O.; Reamon-Buettner, S.M.; Hurek, T.; Reinhold-Hurek, B.


FEMS microbiology letters 249 (2005), No.2, pp.233-240
ISSN: 0378-1097
Journal Article
Fraunhofer ITEM ()

Azoarcus sp. strain BH72 is a Gram-negative proteobacterium of the subclass; it is a diazotrophic endophyte of graminaceous plants and can provide significant amounts of fixed nitrogen to its host plant Kallar grass. We aimed to obtain a physical map of the Azoarcus sp. strain BH72 chromosome to be directly used in functional analysis and as a part of an Azoarcus sp. BH72 genome project. A bacterial artificial chromosome (BAC) library was constructed and analysed. A representative physical map with a high density of marker genes was developed in which 64 aligned BAC clones covered almost the entire genome.