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New method for confocal microscopy and its endoscopic application

: Krohne, I.; Pfeifer, T.; Bitte, F.; Zacher, M.; Meier, R.


Boccara, A.-C. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.; Optical Society of America -OSA-, Washington/D.C.:
Novel optical instrumentation for biomedical applications : 24 - 25 June 2003, Munich, Germany; Conference on Novel Optical Instrumentation for Biomedical Applications, part of the SPIE/OSA Biomedical Optics Europe Meeting
Bellingham, WA: SPIE, 2003 (Proceedings of SPIE 5143)
ISBN: 978-0-8194-5013-5
ISBN: 0-8194-5013-8
ISSN: 1605-7422
Conference on Novel Optical Instrumentation for Biomedical Applications <2003, München>
European Conference on Biomedical Optics <2003, München>
Conference Paper
Fraunhofer IPT ()

Today, confocal microscopy is widely used in biomedical applications, since its technology allows e.g. in cell biology to resolve sub- and intercellular details in three-dimensions. Moreover, the recent miniaturization process of opto-electronical components even indicates a possible endoscopic application - to intraoperative examinations without need for a surgical biopsy. The current development of such a confocal endomicroscope, based on the concept of miniaturized fiberoptical components is described. The technical setup is explained in the context of an alternative method for confocal microscopy employing a Digital Micromirror Device (DMD). Moreover, in-vivo tissue staining techniques suitable for confocal endoscopic imaging are analyzed.