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Localization and topology of ratp28, a member of a novel family of putative steroid-binding proteins

 
: Nölte, I.; Jeckel, D.; Wieland, F.T.; Sohn, K.

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Biochimica et biophysica acta. Protein structure and molecular enzymology 1543 (2000), No.1, pp.123-130
ISSN: 0167-4838
English
Journal Article
Fraunhofer IGB ()

Abstract
We have cloned ratp28, a membrane protein from rat liver homologous to the previously described hpr6.6, a putative steroid-binding protein in humans. Ratp28 has a type II topology as determined by protease digestion experiments on intact and detergent-solubilized membranes. Subcellular fractionation by sucrose density centrifugation revealed a distribution for ratp28 identical to Bip as a marker for membranes of the endoplasmic reticulum. In these experiments no association was found with markers for Golgi or plasma membranes, indicating that ratp28 is localized to the endoplasmic reticulum.

: http://publica.fraunhofer.de/documents/N-171554.html