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Methods and instruments for continuous-flow PCR on a chip

: Gärtner, C.; Klemm, R.; Becker, H.


Papautsky, I. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.:
Microfluidics, BioMEMS, and medical microsystems V : 22 - 24 January 2007 San Jose, California, USA
Bellingham, WA: SPIE, 2007 (Proceedings of SPIE 6465)
ISBN: 978-0-8194-6578-8
ISBN: 0-8194-6578-X
Paper 646502
Conference "Microfluidics, BioMEMS, and Medical Microsystems" <5, 2007, San Jose/Calif.>
Conference Paper
Fraunhofer IOF ()

PCR is the most commonly used method to identify DNA segments. Several methods have been used to miniaturize PCR and perform it in a microfluidic chip. A unique approach is the continuous-flow PCR, where the conventional thermocycling is replaced by pumping the sample through a channel which meanders over stationary temperature zones, allowing fast temperature changes in the sample due to the low thermal mass as well as a continuous production of PCR products. In this paper we present a system comprising a polymer microfluidic chip, a thermocycler unit and the protocols necessary to perform fast continuous-flow PCR including experimental results in comparison with a conventional PCR system.