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Characteristics of artificial forisomes from plants and yeast

: Noll, G.A.; Müller, B.; Ernst, A.M.; Rüping, B.; Groscurth, S.; Twyman, R.M.; Kawchuk, L.M.; Prüfer, D.


Bioengineered bugs 2 (2011), No.2, pp.111-114
ISSN: 1949-1018
ISSN: 1949-1026
Journal Article
Fraunhofer IME ()

Forisomes are protein bodies found exclusively in the phloem of the Fabaceae (legumes). In response to wounding, the influx of Ca2+ induces a conformational change from a condensed to a dispersed state which plugs the sieve tubes and prevents the loss of photoassimilates. This reversible, ATP-independent reaction can be replicated with purified forisomes in vitro by adding divalent cations or electrically inducing changes in pH, making forisomes ideal components of technical devices. Although native forisomes comprise several subunits, we recently showed that functional homomeric forisomes with distinct properties can be expressed in plants and yeast, providing an abundant supply of forisomes with tailored properties. Forisome subunits MtSEO-F1 and MtSEO-F4 can each assemble into homomeric artificial forisomes, which indicates functional redundancy. However, we provide further evidence that both proteins are subunits of the native heteromeric forisome body in planta. We also show that the properties of artificial forisomes can be modified by immobilization, which is a prerequisite for their incorporation into technical devices