Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Determination of the turnover number of the restriction endonuclease EcoRI using evanescent wave technology

 
: Bier, F.F.; Kleinjung, F.; Schmidt, P.M.; Scheller, F.W.

Fresenius Journal of Analytical Chemistry 372 (2002), No.2, pp.308-313
ISSN: 0937-0633
English
Journal Article
Fraunhofer IBMT ()

Abstract
Binding and catalytic activity of the type II restriction endonuclease EcoRI on immobilized DNA has been observed in real time using three different evanescent wave biosensors and two different immobilization techniques. The method gives direct access to the turnover number (kcat) without the necessity for the determination of any concentration or activity. The combination of different evanescent wave techniques gives access to the catalytic mechanism and allows the determination of the rate limiting step.

: http://publica.fraunhofer.de/documents/N-170259.html