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PEG-based thermo-responsive polymer coatings for the control of cell adhesion

: Lankenau, A.; Wischerhoff, E.; Uhlig, K.; Börner, H.G.; Lutz, J.F.; Duschl, C.


Dössel, O. ; International Union for Physical and Engineering Sciences in Medicine -IUPESM-:
World Congress on Medical Physics and Biomedical Engineering 2009. Vol.10: Biomaterials, cellular and tissue engineering, artificial organs : 7 - 12 September, 2009, Munich, Germany; WC 2009; 11th international congress of the IUPESM
Berlin: Springer, 2009 (IFMBE proceedings 25/10)
ISBN: 978-3-642-03899-0
ISBN: 978-3-642-03900-3
World Congress on Medical Physics and Biomedical Engineering <2009, München>
Conference Paper
Fraunhofer IAP ()
Fraunhofer IBMT ()

In various fields of biotechnology, the control of cell adhesion to culture substrates is an important issue. To avoid or reduce the utilization of digesting enzymes like trypsin for the efficient removal of cells from surfaces, thermoresponsive polymers have been used which mediate cell adhesion at the common culture temperature of 37°C but repel cells upon incubation at reduced temperatures like 25°C. In particular, the thermo-responsive polymer poly(N-isopropylacrylamide) (PNIPAM) and derivatives thereof have been extensively studied and recently commercialized. We present an alternative solution for the generation of thermo-responsive cell culture surfaces comprising copolymers of 2-(2-methoxyethoxy) ethyl methacrylate (MEO 2MA) and oligo(ethylene glycol) methacrylate (OEGMA). These surfaces expose only functional groups, which are based on poly(ethylene glycol) (PEG) and therefore can be assumed to be particularly biocompatible. The control of cell adhesion was fou nd to be highly efficient.