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Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays

 
: Geißler, D.; Hill, D.; Löhmannsröben, H.-G.; Thomas, E.; Lavigne, A.; Darbouret, B.; Bois, E.; Charbonniere, L.J.; Ziessel, R.F.; Hildebrandt, N.

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Coté, G.L. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.:
Optical diagnostics and sensing X: Toward point-of-care diagnostics : 25 - 26 January 2010, San Francisco, California, United States
Bellingham, WA: SPIE, 2010 (Proceedings of SPIE 7572)
ISBN: 978-0-8194-7968-6
ISSN: 1605-7422
Paper 75720C
Conference "Optical Diagnostics and Sensing" <10, 2010, San Francisco/Calif.>
English
Conference Paper
Fraunhofer IAP ()

Abstract
An optical multiplexed homogeneous (liquid phase) immunoassay based on FRET from a terbium complex to eight different fluorescent dyes is presented. We achieved highly sensitive parallel detection of four different lung cancer specific tumor markers (CEA, NSE, SCC and CYFRA21-1) within a single assay and show a proof-of-principle for 5- fold multiplexing. The method is well suited for fast and low-cost miniaturized point-of-care testing as well as for highthroughput screening in a broad range of in-vitro diagnostic applications.

: http://publica.fraunhofer.de/documents/N-170068.html