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  4. Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays
 
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2010
Conference Paper
Title

Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays

Abstract
An optical multiplexed homogeneous (liquid phase) immunoassay based on FRET from a terbium complex to eight different fluorescent dyes is presented. We achieved highly sensitive parallel detection of four different lung cancer specific tumor markers (CEA, NSE, SCC and CYFRA21-1) within a single assay and show a proof-of-principle for 5- fold multiplexing. The method is well suited for fast and low-cost miniaturized point-of-care testing as well as for highthroughput screening in a broad range of in-vitro diagnostic applications.
Author(s)
Geißler, D.
Hill, D.
Löhmannsröben, H.-G.
Thomas, E.
Lavigne, A.
Darbouret, B.
Bois, E.
Charbonniere, L.J.
Ziessel, R.F.
Hildebrandt, N.
Mainwork
Optical diagnostics and sensing X: Toward point-of-care diagnostics  
Conference
Conference "Optical Diagnostics and Sensing" 2010  
DOI
10.1117/12.842731
Language
English
Fraunhofer-Institut für Angewandte Polymerforschung IAP  
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