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In vitro culture of native jejunal segments for use as 3D intestinal testing system

Poster at 4th Congress on Regenerative Biology and Medicine - BioStar 2010, Stuttgart, Germany, 13.-15. Oktober 2010
: Tordy, D.M.; Schanz, J.E.; Pusch, J.; Schröder, B.; Hirth, T.; Walles, H.

Poster urn:nbn:de:0011-n-1608220 (2.2 MByte PDF)
MD5 Fingerprint: 8b4c6d45444cfddff41cb6b2cb262c0a
Created on: 11.5.2011

2010, 1 Folie
Congress on Regenerative Biology and Medicine (BioStar) <4, 2010, Stuttgart>
Poster, Electronic Publication
Fraunhofer IGB ()
native small intestine; in vitro culture; test system; transplant; perfusion organ culture

Despite various kinds of animal testings used to explore intestinal resorption of drugs after oral uptake there is still no satisfying method established to estimate these processes in a human body. The difficulty is due to species differences as well in metabolic processes as in actions of drugs, that complicate usage of blood concentration or medical surveillance for analysis. We succeeded in obtaining isolated jejunal segments of rats and pigs in vitro. Longer than 5 days cells still showed typical immuno-histological markers of cell types naturally found in the intestine. Longer than 9 days there still was an obvious peristaltic movement in cultured pig intestine. By using isolated jejunal segments of different species for uptake studies under well defined conditions it is more easy to analyse uptake amounts, species differences and other influencing factors. Moreover animal testings could be reduced and replaced.