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Expression of active human blood clotting VIII in mammary gland of transgenic rabbits

: Hiripi, L.; Makovics, F.; Halter, R.; Baranyi, M.; Paul, D.; Carnwath, J.; Bösze, Z.; Niemann, H.


DNA and cell biology 22 (2003), No.1, pp.41-45
ISSN: 0198-0238
ISSN: 1044-5498
Journal Article
Fraunhofer ITEM ()
mammary gland; human blood clotting factor VIII; transgenic animal

Human clotting factor VIII is probably the largest protein to be expressed to date in the mammary gland of a transgenic animal, and it requires extensive posttranslational modification to achieve full biological activity. The mammary gland specific construct mWAP-hFVIII-MT-I was injected into the pronuclei of rabbit zygotes, and three transgenic offspring were obtained. Founder 385 showed germ-line transmission of a single integrated copy, and a homozygous line was established from this animal. The rhFVIII was transcribed and translated exclusively in the mammary gland. The activity of rhFVIII in the rabbit milk ranged from 5 to 8% of that found in normal human plasma. Results indicate the suitability of the transgenic rabbit mammary gland for rhFVIII production.