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Interface properties of cytochrome c on a nano-textured diamond surface

: Yang, N.; Hoffmann, R.; Smirnov, W.; Nebel, C.E.


Diamond and Related Materials 20 (2011), No.2, pp.269-273
ISSN: 0925-9635
Journal Article
Fraunhofer IAF ()

The electron transfer process and the peroxidase activity of native and denatured cytochrome c were investigated on OH-terminated diamond nano-textured surfaces. The nano-textured surfaces with geometrical properties of typical protein dimensions were fabricated by a top-down technology, where diamond nanoparticles are used as etchingmasks. The cyclic voltammogram of native cytochrome c shows an adsorption-controlled, reversible electron transfer process of the heme redox center, which was not detected for denatured cytochrome c. Native cytochrome c denatures in the presence of hydrogen peroxide. Higher peroxidase activity was found on the denatured cytochrome c than on native cytochrome c. The apparent Michaelis-Menten constants Km for denatured cytochrome c and the native cytochrome c were determined to be 0.08 and 0.23 mM, respectively.