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On-chip integrated lensless microscopy module for optical monitoring of adherent growing mammalian cells

: Li, W.; Knoll, T.; Thielecke, H.


Institute of Electrical and Electronics Engineers -IEEE-; IEEE Engineering in Medicine and Biology Society -EMBS-:
32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2010 : August 31 - September 4, 2010. Sheraton Hotel, Buenos Aires, Argentina
New York, NY: IEEE, 2010
ISBN: 978-1-4244-4123-5 (Print)
ISBN: 978-4244-4124-2 (Online)
pp.1012-1015 (Vol.2)
Engineering in Medicine and Biology Society (EMBS International Conference) <32, 2010, Buenos Aires>
Conference Paper
Fraunhofer IBMT ()

Lab-on-a-chip systems are increasingly applied in cell-based assays for toxicology and drug testing. In this paper, an on-chip integrated lensless microscopy module using a direct projection method for optical monitoring of the shadow images of adherent growing mammalian cells is presented. The biological cells are conserved and interfaced by a microfabricated cavity chip with a 1 µm thick silicon nitride (Si3N4) substrate onto the surface of a 5 megapixel CMOS image sensor with 2.2 µm pixel size. The optical resolution of the assembly is estimated by the contact/proximate printing theory from optical lithography. Further characterization is made by imaging microbeads in chips with the Si3N4-membrane as well as in cavity chips with membranes made from dry film resist (DFR, thickness 20, 40 and 60 µm). The module represents a 3 × optical microscope for cell morphology imaging. The function is demonstrated by the growth monitoring of L929 cells cultured in cavity chips with Si3N4 substrate for 2 days and by checking the colorimetric staining of cells with a compromised membrane.