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Vorrichtung und Verfahren zur Passage von fluiden Medien

Apparatus for transporting liquid or liquids from culture vessel and/or storage container into further storage container, comprises over-pressure source or lower pressure source, pressure-tight connecting lines, and liquid cell material
 
: Huchler, R.; Fritsche, M.; Malthan, D.

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Frontpage ()

DE 102009021784 A: 20090518
DE 102009021784 A: 20090518
C12M0001
German
Patent, Electronic Publication
Fraunhofer IPA ()

Abstract
(A1) Die Erfindung betrifft eine Vorrichtung zum Transport von einer Fluessigkeit oder mehreren Fluessigkeiten von mindestens einem Kulturgefaess und/oder mindestens einem Vorratsbehaeltnis in mindestens ein weiteres Vorratsbehaeltnis. Der Transport der Fluessigkeit oder der mehreren Fluessigkeiten erfolgt mit einer Ueberdruckquelle oder Unterdruckquelle und mit druckdichten Verbindungsleitungen, wobei mindestens eine Fluessigkeit Zellmaterial aufweist. Erfindungsgemaess weist der Transport der einen Fluessigkeit oder der mehreren Fluessigkeiten keinen Wechsel zwischen Ueberdruck und Unterdruck oder zwischen Unterdruck und Ueberdruck auf und zudem wird die Druckdifferenz nicht Null.

 

DE 102009021784 A1 UPAB: 20101208 NOVELTY - The apparatus for transporting liquid or liquids from a culture vessel and/or a storage container into a further storage container, comprises an over-pressure source or lower pressure source, pressure-tight connecting lines, and a liquid cell material such as animal origin, where the transportation of the liquid or liquids takes place by pressure difference without change between the overpressure and the lower pressure or between the lower pressure and overpressure and the pressure difference is not zero. The liquid or one of the liquids is buffer solution (5) and/or nutrient medium (7). DETAILED DESCRIPTION - The apparatus for transporting liquid or liquids from a culture vessel and/or a storage container into a further storage container, comprises an over-pressure source or lower pressure source, pressure-tight connecting lines, and a liquid cell material such as animal origin, where the transportation of the liquid or liquids takes place by pressure difference without change between the overpressure and the lower pressure or between the lower pressure and overpressure and the pressure difference is not zero. The liquid or one of the liquids is a buffer solution (5), nutrient medium (7) and/or enzyme solution (6). The applied pressure to the liquid or liquids is reached by an impact with a fluid and a germ-free gas e.g. compressed air (1, 4) or an air, which is enriched with carbon dioxide and/or oxygen. The apparatus has valves that are controlled by a microcontroller. The transportation of the liquid or liquids completely or partially takes place through cavities in a common carrier plate. An INDEPENDENT CLAIM is included for a method for transporting liquid or liquids. USE - Apparatus for transporting liquid or liquids from a culture vessel and/or storage container into a further storage container useful for the production of biological cell material, in the field of biopharmaceutical industry during the development of drugs, for the production of artificial tissues for test systems or in the field of regenerative medicine. ADVANTAGE - The apparatus is capable of flexibly, simply and rapidly transporting the liquid or liquids from the culture vessel and/or storage container into the further storage container with high yield and reliability.

: http://publica.fraunhofer.de/documents/N-148704.html