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Surfactant protein D modulates pulmonary clearance of pollen starch granules

 
: Winkler, C.; Hüper, K.; Wedekind, A-C.; Rochlitzer, S.; Hartwig, C.; Müller, M.; Braun, A.; Krug, N.; Hohlfeld, J.M.; Erpenbeck, V.J.

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Experimental Lung Research 36 (2010), No.9, pp.522-530
ISSN: 0190-2148
ISSN: 1521-0499
English
Journal Article
Fraunhofer ITEM ()
optical imaging; T-cell proliferation; allergen; dendritic cell; macrophages

Abstract
Pollen starch granules (PSGs) are allergen particles that get into contact with pulmonary surfactant and phagocytes in the terminal airways. In this study, the effects of surfactant protein D (SP-D) on the interaction of PSGs with phagocytes and on the pulmonary clearance of PSGs were determined. Fluorescently labeled PSGs were incubated in vitro with murine lung macrophages or dendritic cells (DCs) +/- recombinant rat SP-D (rrSP-D). In addition, the effect of SP-D on uptake of PSGs by lung macrophages and DCs was studied in vivo. Furthermore, PSGs were instilled in Balb/c mice and the effects of SP-D on total lung clearance were assessed by optical imaging. SP-D treatment increased the number of PSG-positive macrophages and DCs in vitro. Furthermore, SP-D accelerated uptake/binding by alveolar macrophages and reduced the number of PSG-positive tissue macrophages and DCs at 24 hours. However, SP-D did not affect total lung clearance of PSGs and it did not enhance the T-cell proliferation induced by PSG-positive DCs. In conclusion, SP-D increased PSG-positive cells in vitro and accelerated PSG binding/uptake in vivo. The observed effects were limited to cellular clearance mechanisms and did not affect the total clearance of PSGs from the lung.

: http://publica.fraunhofer.de/documents/N-146488.html