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Sensor fuer elektrophysiologische Untersuchungen an lebenden Zellen und Verfahren zu seiner Herstellung

Sensor for electrophysiological investigation in living cells, comprises electrodes, which are formed in a row-or matrix arrangement on the surface of an electrically isolated substrate and are electrically isolated from one another
 
: Meyer-Plath, A.; Jäger, M.; Sonntag, F.

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Frontpage ()

DE 102008056277 A: 20081028
DE 102008056277 A: 20081028
C12Q0001
German
Patent, Electronic Publication
Fraunhofer IWS ()

Abstract
(A1) Die Erfindung betrifft Sensoren fuer elektrophysiologische Untersuchungen an lebenden Zellen und ein Verfahren zur Herstellung solcher Sensoren. Aufgabe der Erfindung ist es Sensoren fuer elektrophysiologische Untersuchungen an lebenden Zellen zur Verfuegung zu stellen, deren Messgenauigkeit erhoeht ist und mit denen reproduzier- und vergleichbare Untersuchungsergebnisse erhalten werden koennen. Bei dem erfindungsgemaessen Sensor sind Elektroden in einer Reihen- oder Matrixanordnung auf der Oberflaeche eines elektrisch isolierenden Substrats ausgebildet und durch den polymeren Substratwerkstoff oder einen zwischen den Elektroden auf der Substratoberflaeche aufgebrachten polymeren Werkstoff elektrisch voneinander isoliert. Die Oberflaeche eines polymeren Werkstoffs oder polymeren Substratwerkstoffs ist dabei oertlich gezielt modifiziert, so dass Oberflaechenbereiche mit erhoehter Adhaesion fuer Zellen zwischen Elektroden vorhanden sind.

 

DE 102008056277 A1 UPAB: 20100524 NOVELTY - The sensor comprises electrodes (5), which are formed in a row-or matrix arrangement on the surface of an electrically isolated substrate (1) and are electrically isolated from one another through polymer substrate material or a polymer material subjected between the electrodes on the substrate surface. The surface of the polymer material or polymer substrate material is modified so that the surface areas (4) are equipped with increased adhesion for cells between electrodes. The additional surface areas of the polymer material are locally hydrophobized. DETAILED DESCRIPTION - The sensor comprises electrodes (5), which are formed in a row-or matrix arrangement on the surface of an electrically isolated substrate (1) and are electrically isolated from one another through polymer substrate material or a polymer material subjected between the electrodes on the substrate surface. The surface of the polymer material or polymer substrate material is modified so that the surface areas (4) are equipped with increased adhesion for cells between electrodes. The additional surface areas of the polymer material are locally hydrophobized. The surface is modified with carboxyl-, hydroxyl-, and/or amino groups or with fluorine compound. The electrodes and modified surface areas are arranged within a flow channel for culture medium and/or sample fluid. The row arrangement of the electrodes is available, so that the cells associated to a row are influenceable through a flow channel formation respectively with different culture medium or different sample fluid. The surface areas are formed as gradient field. An INDEPENDENT CLAIM is included for a method for the production of a sensor for electrophysiological investigation at living cells. USE - Sensor useful for electrophysiological investigation in living cells. ADVANTAGE - The sensor ensures simple, efficient and accurate electrophysiological investigation in living cells.

: http://publica.fraunhofer.de/documents/N-139577.html