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Gel-based biochip for the detection of airborne contaminants

: Schmitt, K.; Sulz, G.; Klockenbring, T.; Seidel, B.; Holländer, A.

Preprint urn:nbn:de:0011-n-1371780 (759 KByte PDF)
MD5 Fingerprint: 47c1f4008ec43707cf6ab41a5b281cca
The original publication is available at
Created on: 13.8.2010

Microsystem Technologies 16 (2010), No.5, pp.717-722
ISSN: 0946-7076
Journal Article, Electronic Publication
Fraunhofer IME ()
Fraunhofer IPM ()
Fraunhofer IAP ()
indoor air quality; microorganism; immunobiological recognition; fluorescence; polymer substrate; electrophoretic separation

The fast and direct identification of possibly pathogenic microorganisms in air is gaining increasing interest due to their threat for public health, e.g. in clinical environments or in clean rooms of food or pharmaceutical industries. We present a new detection method allowing the direct recognition of relevant germs or bacteria via fluorescence-labeled antibodies in a gel-based biochip within less than 1 h. In detail, the microorganisms are collected on a gel-based substrate containing fluorescence-labeled antibodies for the detection of a specific microorganism. First measurements with the system to identify test particles as well as interfering influences have been performed, in particular with respect to autofluorescence of dust particles. Specific antibodies for the detection of Aspergillus fumigatus spores have been established. The biological test system consists of protein A-coated polymer particles which are detected by a fluorescence-labeled IgG. Furthermore the influence of interfering particles such as dust or debris is discussed.