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Fc gamma receptor-mediated antigen uptake by lung DC contributes to allergic airway hyper-responsiveness and inflammation

 
: Hartwig, C.; Mazzega, M.; Constabel, H.; Krishnaswamy, J.K.; Gessner, J.E.; Braun, A.; Tschernig, T.; Behrens, G.M.N.

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European Journal of Immunology 40 (2010), No.5, pp.1284-1295
ISSN: 0014-2980
ISSN: 1521-4141
English
Journal Article
Fraunhofer ITEM ()
antigen presentation/processing; DC; Fc gamma receptor; Asthma; inflammation

Abstract
During asthma, lung DC capture and process antigens to initiate and maintain allergic Th2 cell responses to inhaled allergens. The aim of the study was to investigate whether allergen-specific IgG, generated during sensitization, can potentiate the acute airway inflammation through Fc gamma receptor (Fc gamma R)-mediated antigen uptake and enhance antigen presentation resulting in augmented T-cell proliferation. We examined the impact of antigen presentation and T-cell stimulation on allergic airway hyperresponsiveness and inflammation using transgenic and gene-deficient mice. Both airway inflammation and eosinophilia in bronchoalveolar lavage fluid were markedly reduced in sensitized and challenged Fc gamma R-deficient mice. Lung DC of WT, but not Fc gamma R-deficient mice, induced increased antigen-specific CD4(+) T-cell proliferation when pulsed with anti-OVA IgG immune complexes. Intranasal application of anti-OVA IgG immune complexes resulted in enhanced airway inflammation, eosinophilia and Th2 cytokine release, mediated through enhanced antigen-specific T-cell proliferation in vivo. Finally, antigen-specific IgG in the serum of sensitized mice led to a significant increase of antigen-specific CD4(+) T-cell proliferation induced by WT, but not Fc gamma R-deficient, lung DC. We conclude that Fc gamma R-mediated enhanced antigen presentation and T-cell stimulation by lung DC has a significant impact on inflammatory responses following allergen challenge in asthma.

: http://publica.fraunhofer.de/documents/N-136739.html