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Allergen particle binding by human primary bronchial epithelial cells is modulated by surfactant protein D

 
: Schleh, C.; Erpenbeck, V.J.; Winkler, C.; Lauenstein, H.D.; Nassimi, M.; Braun, A.; Krug, N.; Hohlfeld, J.M.

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Postprint urn:nbn:de:0011-n-1345440 (739 KByte PDF)
MD5 Fingerprint: c5d5035c5f4bc2cd51d31e05a7a6e6a4
Created on: 15.7.2010


Respiratory research. Online journal 11 (2010), Art. 83, 33 pp.
http://respiratory-research.com/
ISSN: 1465-993X
ISSN: 1465-9921
English
Journal Article, Electronic Publication
Fraunhofer ITEM ()

Abstract
Background
Allergen-containing subpollen particles (SPP) are released from whole plant pollen upon contact with water or even high humidity. Because of their size SPP can preferentially reach the lower airways where they come into contact with surfactant protein (SP)-D. Our previous work demonstrated that SP-D increases the uptake of SPP by alveolar macrophages. In the present study, we investigated the uptake of SPP in human primary epithelial cells and the potential modulation by SP-D. The patho-physiological consequence was evaluated by measurement of pro-inflammatory mediators.
Methods
SPP were isolated from timothy grass and subsequently fluorescently labelled. Human primary bronchial epithelial cells were incubated with SPP or polystyrene particles (PP) in the presence and absence of surfactant protein D. In addition, different sizes and surface charges of the PP were studied. Particle uptake was evaluated by flow cytometry and confocal microscopy. Soluble mediators were measured by enzyme linked immunosorbent assay or bead array.
Results
SPP were taken up by primary epithelial cells in a dose dependent manner. This uptake was coincided with secretion of Interleukin (IL)-8. SP-D increased the fraction of bronchial epithelial cells that bound SPP but not the fraction of cells that internalized SPP. SPP-induced secretion of IL-8 was further increased by SP-D. PP were bound and internalized by epithelial cells but this was not modulated by SP-D.
Conclusions
Epithelial cells bind and internalize SPP and PP which leads to increased IL-8 secretion. SP-D promotes attachment of SPP to epithelial cells and may thus be involved in the inflammatory response to inhaled allergen.

: http://publica.fraunhofer.de/documents/N-134544.html