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Detection of angiotensin II type 1 receptor ligands by a cell-based assay

: Grießner, M.; Bröker, P.; Lehmann, A.; Ehrentreich-Förster, E.; Bier, F.F.


Analytical and bioanalytical chemistry 395 (2009), No.6, pp.1937-1940
ISSN: 1618-2642 (Print)
ISSN: 1618-2650 (Online)
Journal Article
Fraunhofer IBMT ()

This work describes a cell-based assay that does not depend on radioactivity or laboratory animals for the detection of ligands of angiotensin II type 1 receptor (AT(1)R). The assay makes use of stable transfected Chinese hamster ovary cells (CHO-AT(1)R) expressing the AT(1)R. A sequential saturation assay principle was used in which receptor binding sites of the CHO-AT(1)R cells are blocked by the analyte in a concentration-dependent manner. Afterwards, TAMRA-angiotensin II, a fluorescence-labeled ligand, was added to bind to the remaining free binding sites of the receptor. In consequence, the fluorescence signal determined is inversely proportional to the concentration of the analyte.