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Novel lab-on-a-chip system for the label-free detection of DNA hybridization and protein-protein interaction by surface plasmon resonance (SPR)

: Sonntag, F.; Schmieder, S.; Danz, N.; Mertig, M.; Schilling, N.; Klotzbach, U.; Beyer, E.


Rodríguez-Vázquez, A.B. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Europe, Cardiff:
Bioengineered and bioinspired systems IV. Proceedings : 4 May 2009, Dresden, Germany
Bellingham, WA: SPIE, 2009 (SPIE Proceedings 7365)
ISBN: 978-0-8194-7639-5
ISBN: 0-8194-7639-0
Paper 73650Q, 9 pp.
Conference "Bioengineered and Bioinspired Systems" <4, 2009, Dresden>
Conference Paper
Fraunhofer IWS ()
BioMEMS; Bio-Optics; blood; DNA; injection moulding; Lab-on-a-Chip; microfluidics; micro-optics; molecular-biophysics; protein; soft lithography; surface plasmon resonance; temperature control

The detection of specific DNA sequences for the analysis of mutations as well as the detection of proteins gains increasing importance in the field of point-of-care diagnostics. Here, a novel low-cost lab-on-a-chip system for label-free detection of DNA hybridization and protein-protein interaction is introduced. The platform consists of a reader with disposable SPR chips produced by injection moulding. Micro optical elements are integrated into the chip to accomplish a simple connection to the optical reader. Automated, software-controlled reagent handling is achieved by a temperature-controlled microfluidic system comprising a syringe pump and a switching valve. The sensing area can be separated into maximum 40 parts for parallel analysis. Patterned functionalization is achieved by inverse micro contact printing. Several application examples, ranging from on-chip DNA hybridization up to the detection of antibodies inside diluted human blood serum, will be demonstrated.