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Suspension characterization as important key for toxicological investigations

: Meißner, T.; Potthoff, A.; Richter, V.


Nanosafe 2008, International Conference on Safe production and use of nanomaterials : 3-7 November 2008, Minatec, France
Bristol: IOP Publishing, 2009 (Journal of physics. Conference series 170.2009)
ISSN: 1742-6588
Art. 012012, 6 pp.
International Conference on Safe Production and Use of Nanomaterials (Nanosafe) <2008, Minatec>
Conference Paper
Fraunhofer IKTS ()
biological technique; chemical hazard; health hazard; nanoparticle; suspension; titanium compounds; toxicology; tungsten compound

To assess potential health risks of nanoparticles by means of in vitro or in vivo assays and to determine dose-action curves a defined and reproducible method of particle administration is required. The interpretation of the toxicological results should be based on a comprehensive chemical-physical characterization of the particles used. Therefore, we developed a method to suspend nanoparticles stably and homogenously in physiological media. Our approach consist of three steps: (1) physical-chemical characterisation of the powders as delivered, (2) preparation and characterization of a non-physiological electro-statically stabilized nanoparticle suspension and (3) assessment of the nanoparticles behaviour in physiological media with or without proteins. This approach is demonstrated on a t itanium dioxide and a tungsten carbide nanopowder. Results showed that particles agglomerate in protein-free medium within minutes, whereas in the presence of bovine serum albumin or foetal bovine serum an agglomeration is hindered.