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2001
Conference Paper
Titel
Effects of NeemAzal on fish
Titel Supplements
Full life cycle test with the zebrafish, Danio rerio
Abstract
A study was conducted in order to evaluate the effects of NeemAzal (a standardised variant of the active ingredient Azadirachtin) on different life stages and generations of zebrafish (Danio rerio) during a full life cycle, including reproduction. The study provides data being relevant for the assessment of effects on populations. This report deals with the complete life cycle test, including an early life stage test of the FI-generation (day 1 - day 37), investigations on survival, growth and reproduction of FI-generation from day 38 to day 135, as well as an early life stage test of the FII-generation (day 135 - day 174). Lethal and sublethal effects were assessed and compared with control values to determine the lowest observed effect concentration (LOEC) and hence the no observed effect concentration (NOEC). Early life stage tests with FI and FII-generation were carried out in accordance with the OECD guideline 210 (fish, early life stage toxicity test (1)). In the study plan of this life cycle test a conception was attempted which may qualify as a general approach to evaluating the effects of substances on the population of fish and their development. The results show that the conception may well serve as guideline. During the course of the study the concentration of active ingredient was monitored at regular intervals analytically. The investigated NeemAzal concentrations were (mg/L): 0 (control), 0.2, 0.63, 2.0 and 6.4, respectively. According to the obtained results and following the model of a concentration-response-relationship, the following effect concentrations can be stated for the complete full life cycle test (174 days) with zebrafish and NeemAzal as test substance.
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