Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.
2019Critical analysis of the commercial potential of plants for the production of recombinant proteins
Schillberg, S.; Raven, N.; Spiegel, H.; Rasche, S.; Buntru, M.
Journal Article
2019The immunome of soy bean allergy: Comprehensive identification and characterization of epitopes
Kern, Karolin; Havenith, Heide; Delaroque, Nicolas; Rautenberger, Paul; Lehmann, Jörg; Fischer, Markus; Spiegel, Holger; Schillberg, Stefan; Ehrentreich-Förster, Eva; Aurich, Stefanie; Treudler, Regina; Szardenings, Michael
Journal Article
2019Ready-to-Use Stocks of Agrobacterium tumefaciens can Simplify Process Development for the Production of Recombinant Proteins by Transient Expression in Plants
Spiegel, H.; Boes, A.; Perales Morales, C.; Rademacher, T.; Buyel, J.F.
Journal Article
2019Simplified tracking of a soy allergen in processed food using a monoclonal antibody-based sandwich ELISA targeting the soybean 2S albumin gly m 8
Ueberham, Elke; Spiegel, Holger; Havenith, Heide; Rautenberger, Paul; Lidzba, Norbert; Schillberg, Stefan; Lehmann, Jörg
Journal Article
2018Aspergillus-specific antibodies: Targets and applications
Schubert, M.; Spiegel, H.; Schillberg, S.; Nölke, G.
Journal Article
2018Current status and perspectives of the molecular farming landscape
Spiegel, H.; Stöger, E.; Twyman, R.M.; Buyel, J.F.
Book Article
2018Downstream processing of a plant-derived malaria transmission-blocking vaccine candidate
Menzel, S.; Holland, T.; Boes, A.; Spiegel, H.; Fischer, R.; Buyel, J.F.
Journal Article
2018Proteomic analysis of CHO cell lines producing high and low quantities of a recombinant antibody before and after selection with methotrexate
Hausmann, R.; Chudobová, I.; Spiegel, H.; Schillberg, S.
Journal Article
2017Combination of two epitope identification techniques enables the rational design of soy allergen Gly m 4 mutants
Havenith, Heide; Kern, Karolin; Rautenberger, Paul; Spiegel, Holger; Szardenings, Michael; Ueberham, Elke; Lehmann, Jörg; Buntru, Matthias; Vogel, Simon; Treudler, Regina; Fischer, Rainer; Schillberg, Stefan
Journal Article
2017Immunization with the Malaria Diversity-Covering Blood-Stage Vaccine Candiate Plasmodium falciparum Apical Membrane Antigen 1 DiCo in Complex with Its Natural Ligand PfRpm2 Does Not Improve the In Vitro Efficacy
Spiegel, H.; Boes, A.; Fendel, R.; Reimann, A.; Schillberg, S.; Fischer, R.
Journal Article
2017Improvement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris
Kastilan, R.; Boes, A.; Spiegel, H.; Voepel, N.; Chudobova, I.; Hellwig, S.; Buyel, J.F.; Reimann, A.; Fischer, R.
Journal Article
2017Soybean allergy related epitopes
Szardenings, Michael; Kern, Karolin; Spiegel, Holger; Havenith, Heide
Patent
2016Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail
Boes, A.; Spiegel, H.; Kastilan, R.; Bethke, S.; Voepel, N.; Chudobova, I.; Bolscher, J.M.; Dechering, K.J.; Fendel, R.; Buyel, J.F.; Reimann, A.; Schillberg, S.; Fischer, R.
Journal Article
2016Characterization of a novel inhibitory human monoclonal antibody directed against Plasmodium falciparum Apical Membrane Antigen 1
Maskus, D.J.; Królik, M.; Bethke, S.; Spiegel, H.; Kapelski, S.; Seidel, M.; Addai-Mensah, O.; Reimann, A.; Klockenbring, T.; Barth, S.; Fischer, R.; Fendel, R.
Journal Article
2016Development of a competitive cystatin C-specific bioassay suitable for repetitive measurements
Damm, T.; Spiegel, H.; Barth, S.; Fischer, R.; Naehring, J.
Journal Article
2016Optimized blanching reduces the host cell protein content and substantially enhances the recovery and stability of two plant-derived malaria vaccine candidates
Menzel, S.; Holland, T.; Boes, A.; Spiegel, H.; Bolzenius, J.; Fischer, R.; Buyel, J.F.
Journal Article
2016A Plant-Based Transient Expression System for the Rapid Production of Malaria Vaccine Candidates
Boes, A.; Reimann, A.; Twyman, R.M.; Fischer, R.; Schillberg, S.; Spiegel, H.
Book Article
2015Analysis of a multi-component multi-stage malaria vaccine candidate-tackling the cocktail challenge
Boes, A.; Spiegel, H.; Voepel, N.; Edgue, G.; Beiss, V.; Kapelski, S.; Fendel, R.; Scheuermayer, M.; Pradel, G.; Bolscher, J.M.; Behet, M.C.; Dechering, K.J.; Hermsen, C.C.; Sauerwein, R.W.; Schillberg, S.; Reimann, A.; Fischer, R.
Journal Article
2015Application of a scalable plant transient gene expression platform for malaria vaccine development
Spiegel, H.; Boes, A.; Voepel, N.; Beiss, V.; Edgue, G.; Rademacher, T.; Sack, M.; Schillberg, S.; Reimann, A.; Fischer, R.
Journal Article
2015Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits
Boes, A.; Spiegel, H.; Edgue, G.; Kapelski, S.; Scheuermayer, M.; Fendel, R.; Remarque, E.; Altmann, F.; Maresch, D.; Reimann, A.; Pradel, G.; Schillberg, S.; Fischer, R.
Journal Article
2015Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody
Kapelski, S.; Boes, A.; Spiegel, H.; Almeida, M. de; Klockenbring, T.; Reimann, A.; Fischer, R.; Barth, S.; Fendel, R.
Journal Article
2015From gene to harvest: Insights into upstream process development for the GMP production of a monoclonal antibody in transgenic tobacco plants
Sack, M.; Rademacher, T.; Spiegel, H.; Boes, A.; Hellwig, S.; Drossard, J.; Stoger, E.; Fischer, R.
Journal Article
2015Heat-precipitation allows the efficient purification of a functional plant-derived malaria transmission-blocking vaccine candidate fusion protein
Beiss, V.; Spiegel, H.; Boes, A.; Kapelski, S.; Scheuermayer, M.; Edgue, G.; Sack, M.; Fendel, R.; Reimann, A.; Schillberg, S.; Pradel, G.; Fischer, R.
Journal Article
2015Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10
Maskus, DJ.; Bethke, S.; Seidel, M.; Kapelski, S.; Addai-Mensah, O.; Boes, A.; Edgu, G.; Spiegel, H.; Reimann, A.; Fischer, R.; Barth, S.; Klockenbring, T.; Fendel, R.
Journal Article
2015Optimization of a multi-stage, multi-subunit malaria vaccine candidate for the production in Pichia pastoris by the identification and removal of protease cleavage sites
Spiegel, H.; Schinkel, H.; Kastilan, R.; Dahm, P.; Boes, A.; Scheuermayer, M.; Chudobova, I.; Maskus, D.; Fendel, R.; Schillberg, S.; Reimann, A.; Fischer, R.
Journal Article
2015Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50
Beiss, V.; Spiegel, H.; Boes, A.; Scheuermayer, M.; Reimann, A.; Schillberg, S.; Fischer, R.
Journal Article
2015The stage-specific in vitro efficacy of a malaria antigen cocktail provides valuable insights into the development of effective multi-stage vaccines
Spiegel, H.; Boes, A.; Kastilan, R.; Kapelski, S.; Edgue, G.; Beiss, V.; Chubodova, I.; Scheuermayer, M.; Pradel, G.; Schillberg, S.; Reimann, A.; Fischer, R.
Journal Article
2015A versatile coupled cell-free transcription–translation system based on tobacco BY-2 cell lysates
Buntru, M.; Vogel, S.; Stoff, K.; Spiegel, H.; Schillberg, S.
Journal Article
2014An immunohistochemical assay on human tissue using a human primary antibody
Peuscher, A.; Gassler, N.; Schneider, U.; Thom, P.; Rasche, S.; Spiegel, H.; Schillberg, S.
Journal Article
2014Malaria vaccine candidate antigen targeting the pre-erythrocytic stage of Plasmodium falciparum produced at high level in plants
Voepel, N.; Boes, A.; Edgue, G.; Beiss, V.; Kapelski, S.; Reimann, A.; Schillberg, S.; Pradel, G.; Fendel, R.; Scheuermayer, M.; Spiegel, H.; Fischer, R.
Journal Article
2014Tobacco BY-2 cell-free lysate: An alternative and highly-productive plant-based in vitro translation system
Buntru, M.; Vogel, S.; Spiegel, H.; Schillberg, S.
Journal Article
2013Plant-based production of recombinant plasmodium surface protein Pf38 and evaluation of its potential as a vaccine candidate
Feller, Tanja; Thom, Pascal; Koch, Natalie; Spiegel, Holger; Addai-Mensah, Otchere; Fischer, Rainer; Reimann, Andreas; Pradel, Gabriele; Fendel, Rolf; Schillberg, Stefan; Scheuermayer, Matthias; Schinkel, Helga
Journal Article
2013Protective oral vaccination against Infectious bursal disease virus using the major viral antigenic protein VP2 produced in Pichia pastoris
Taghavian, O.; Spiegel, H.; Hauck, R.; Hafez, H.M.; Fischer, R.; Schillberg, S.
Journal Article
2012A potential nanobiotechnology platform based on infectious bursal disease subviral particles
Taghavian, Omid; Mandal, Manoj K.; Steinmetz, Nicole F.; Rasche, Stefan; Spiegel, Holger; Fischer, Rainer; Schillberg, Stefan
Journal Article
2011Affinity purification of a framework 1 engineered mouse/human chimeric IgA2 antibody from tobacco
Boes, A.; Spiegel, H.; Delbrück, H.; Fischer, R.; Schillberg, S.; Sack, M.
Journal Article
2011Characterization of the single-chain Fv-Fc antibody MBP10 produced in Arabidopsis alg3 mutant seeds
Henquet, M.; Eigenhuijsen, J.; Hesselink, T.; Spiegel, H.; Schreuder, M.; Duijn, E. van; Cordewener, J.; Depicker, A.; Krol, A. van der; Bosch, D.
Journal Article
2008Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit
Chung, W.Y.; Sack, M.; Carter, R.; Spiegel, H.; Fischer, R.; Hirst, T.R.; Williams, N.A.; James, R.F.L.
Journal Article
2005DURCH MOLEKULARES PATHOGENICID VERMITTELTE KRANKHEITSRESISTENZ IN PFLANZEN
Fischer, R.; Schillberg, S.; Naehring, J.; Sack, M.; Monecke, M.; Liao, Y.; Spiegel, H.; Zimmerman, S.; Emans, N.; Holzem, A.
Patent
2005Four-dimensional imaging of transvacuolar strand dynamics in tobacco BY-2 cells
Ruthardt, N.; Gulde, N.; Spiegel, H.; Fischer, R.; Emans, N.
Journal Article
2005Generation of human antibody fragments against Streptococcus mutans using a phage display chain shuffling approach
Küpper, M.B.; Huhn, M.; Spiegel, H.; Ma, J.K.-C.; Barth, S.; Fischer, R.; Finnern, R.
Journal Article
2003Using plants as a production system for N-Glycosylated proteins of therapeutic relevance
Hellwig, S.; Sack, M.; Spiegel, H.; Drossard, J.; Fischer, R.
Journal Article
2002Design, assembly and characterization of a humanized scFv framework library
Spiegel, H.; Sack, M.; Finnern, R.; Fischer, R.
Presentation
1999Accumulation of antibody fusion proteins in the cytoplasm and ER of plant cells
Spiegel, H.; Schillberg, S.; Sack, M.; Holzem, A.; Nähring, J.; Monecke, M.; Liao, Y.C.; Fischer, R.
Journal Article