Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.
2019Cell-free synthesis of human toll-like receptor 9 (TLR9): Optimization of synthesis conditions and functional analysis
Dondapati, S.K.; Pietruschka, G.; Thoring, L.; Wüstenhagen, D.A.; Kubick, S.
Journal Article
2019Eukaryotic cell-free systems: A novel platform technology for production and functional characterization of pore-forming toxins
Ramm, F.; Dondapati, S.K.; Zemella, A.; Thoring, L.; Stech, M.; Kubick, S.
Abstract
2019Functional reconstitution of membrane proteins derived from eukaryotic cell-free systems
Dondapati, S.K.; Lübberding, H.; Zemella, A.; Thoring, L.; Wüstenhagen, D.A.; Kubick, S.
Journal Article
2018Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin
Zemella, A.; Thoring, L.; Hoffmeister, C.; Samalikova, M.; Ehren, P.; Wüstenhagen, D.A.; Kubick, S.
Journal Article
2018Cell‐free production of pore forming toxins
Dondapati, S.K.; Wüstenhagen, D.A.; Strauch, E.; Kubick, S.
Journal Article
2018Functional analysis of membrane proteins produced by cell-free translation
Dondapati, S.K.; Wüstenhagen, D.A.; Kubick, S.
Book Article
2018Versatile cell-free protein synthesis systems based on chinese hamster ovary cells
Thoring, L.; Kubick, S.
Book Article
2017Cell-free synthesis of functional antibodies using a coupled in vitro transcription - translation system based on CHO cell lysates
Stech, M.; Nikolaeva, O.; Thoring, L.; Stöcklein, W.F.M.; Wüstenhagen, D.A.; Hust, M.; Dübel, S.; Kubick, S.
Journal Article
2017Comparison of cell-based versus cell-free mammalian systems for the production of a recombinant human bone morphogenic growth factor
Jérôme, V.; Thoring, L.; Salzig, D.; Kubick, S.; Freitag, R.
Journal Article
2017Erratum zu: Neue Systeme zur Antikörperherstellung
Stech, M.; Nikolaeva, O.; Kubick, S.
Journal Article
2017High-yield production of "difficult-to-express" proteins in a continuous exchange cell-free system based on CHO cell lysates
Thoring, L.; Dondapati, S.K.; Stech, M.; Wüstenhagen, D.A.; Kubick, S.
Journal Article
2017Neue Systeme zur Antikörperherstellung
Stech, M.; Nikolaeva, O.; Kubick, S.
Journal Article
2017Production of G protein-coupled receptors in an insect-based cell-free system
Sonnabend, A.; Spahn, V.; Stech, M.; Zemella, A.; Stein, C.; Kubick, S.
Journal Article
2017Qualifying a eukaryotic cell-free system for fluorescence based GPCR analyses
Zemella, Anne; Grossmann, Solveig; Sachse, Rita; Sonnabend, Andrei; Schäfer, Michael; Kubick, Stefan
Journal Article
2016Cell-free synthesis of functional human epidermal growth factor receptor
Quast, Robert B.; Ballion, Biljana; Stech, Marlitt; Sonnabend, Andrei; Varga, Balázs R.; Wüstenhagen, Doreen A.; Kele, Péter; Schiller, Stefan M.; Kubick, Stefan
Journal Article
2016Cell-free systems based on CHO cell lysates
Thoring, Lena; Wüstenhagen, Doreen A.; Borowiak, Maria; Stech, Marlitt; Sonnabend, Andrei; Kubick, Stefan
Journal Article
2016hERG ion channel activity measured in planar lipid bilayer arrays: Reconstitution from eukaryotic cell-free expression system and cellular membrane preparations
Zaitseva, Ekaterina; Rio Martinez, Juan del; Dondapati, Srujan Kumar; Baaken, Gerhard; Petersen, Sönke; Kubick, Stefan; Behrends, Jan C.
Abstract
2016High-yield cell-free synthesis of human EGFR by IRES-mediated protein translation in a continuous exchange cell-free reaction format
Quast, Robert B.; Sonnabend, Andrei; Stech, Marlitt; Wüstenhagen, Doreen A.; Kubick, Stefan
Journal Article
2016Investigation of Factors Influencing Recombinant Human BMP2 Expression in Mammalian cells
Jérôme, V.; Thoring, L.; Salzig, D.; Kubick, S.; Freitag, R.
Journal Article
2016On-chip automation of cell-free protein synthesis
Georgi, V.; Georgi, Leopold; Blechert, Martin; Bergmeister, Merlin; Zwanzig, M.; Wüstenhagen, Doreen A.; Bier, Frank F.; Jung, Erik; Kubick, Stefan
Journal Article
2015Automated production of functional membrane proteins using eukaryotic cell-free translation systems
Quast, Robert B.; Kortt, Oliver; Henkel, Jörg; Dondapati, Srujan K.; Wüstenhagen, Doreen A.; Stech, Marlitt; Kubick, Stefan
Journal Article
2015Cell-free protein synthesis systems derived from cultured mammalian cells
Brödel, A.K.; Wüstenhagen, D.A.; Kubick, S.
Book Article
2015Cell-free protein synthesis: Pros and cons of prokaryotic and eukaryotic systems. Review
Zemella, Anne; Thoring, Lena; Hoffmeister, Christian; Kubick, Stefan
Journal Article
2015Cell-free Synthesis and characterization of difficult-to-express proteins based on eukaryotic cell extracts
Mascher, C.; Aktories, K.; Wüstenhagen, D.; Kubick, S.
Conference Paper, Journal Article
2015Cell-free synthesis meets antibody production
Stech, Marlitt; Kubick, Stefan
Journal Article
2015Characterization of trh2 harbouring vibrio parahaemolyticus strains isolated in Germany
Bechlars, Silke; Jäckel, Claudia; Diescher, Susanne; Wüstenhagen, Doreen A.; Kubick, Stefan; Dieckmann, Ralf; Strauch, Eckhard
Journal Article
2015Cotranslational incorporation of non-standard amino acids using cell-free protein synthesis
Quast, Robert B.; Mrusek, Devid; Hoffmeister, Christian; Sonnabend, Andrei; Kubick, Stefan
Journal Article
2015Messvorrichtung und Fluidikvorrichtung zum Messen einer Menge einer zu untersuchenden Substanz
Jung, Erik; Georgi, Leopold; Kubick, Stefan
Patent
2015Method for producing polyclonal antibodies using an antigenic composition comrising protein-containing membrane vesicles
Kubick, Stefan; Stech, Marlitt; Hanack, Katja; Messerschmidt, Katrin
Patent
2015Templatoptimierung in der zellfreien Proteinsynthese
Sonnabend, A.; Nikolaeva, O.; Kubick, S.
Journal Article
2014Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments
Stech, Marlitt; Hust, Michael; Schulze, Corina; Dübel, Stefan; Kubick, Stefan
Journal Article
2014Cell-free protein expression based on extracts from CHO cells
Brödel, A.K.; Sonnabend, A.; Kubick, S.
Journal Article
2014Cell-free synthesis of membrane proteins: Tailored cell models out of microsomes
Fenz, S.F.; Sachse, R.; Schmidt, T.; Kubick, S.
Journal Article
2014A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells
Stech, M.; Quast, R.B.; Sachse, R.; Schulze, C.; Wüstenhagen, D.A.; Kubick, S.
Journal Article
2014Editorial: New and synthetic bioproduction systems
Kubick, S.; Zeng, A.-P.
Journal Article
2014Future of biosensors: A personal view
Scheller, Frieder W.; Yarman, Aysu; Bachmann, Till; Hirsch, Thomas; Kubick, Stefan; Renneberg, Reinhard; Schumacher, Soeren; Wollenberger, Ulla; Teller, Carsten; Bier, Frank F.
Book Article
2014Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system
Dondapati, S.K.; Kreir, M.; Quast, R.B.; Wüstenhagen, D.A.; Brüggemann, A.; Fertig, N.; Kubick, S.
Journal Article
2014Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes
Sachse, Rita; Dondapati, Srujan K.; Fenz, Susanne F.; Schmidt, Thorsten; Kubick, Stefan
Journal Article
2014Membranproteinsynthese: Zellfrei geht's schneller!
Sachse, Rita; Quast, Robert B.; Sonnabend, Andrei; Stech, Marlitt; Kubick, Stefan
Journal Article
2014Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems
Quast, R.B.; Claussnitzer, I.; Merk, H.; Kubick, S.; Gerrits, M.
Journal Article
2013Cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus
Bechlars, S.; Wüstenhagen, D.A.; Drägert, K.; Dieckmann, R.; Strauch, E.; Kubick, S.
Journal Article
2013Cell-free systems: Functional modules for synthetic and chemical biology
Stech, M.; Brödel, A.K.; Quast, R.B.; Sachse, R.; Kubick, S.
Book Article
2013Functional evaluation of candidate ice structuring proteins using cell-free expression systems
Brödel, A.K.; Raymond, J.A.; Duman, J.G.; Bier, F.F.; Kubick, S.
Journal Article
2013Functional Evaluation of Canidate Ice Structuring Proteins using Cell-free Expression Systems
Brödel, A.K.; Raymond, J.A.; Duman, J.G.; Bier, F.F.; Kubick, S.
Journal Article
2013IRES-mediated translation of membrane proteins and glycoproteins in eukaryotic cell-free systems
Brödel, A.K.; Sonnabend, A.; Roberts, L.O.; Stech, M.; Wüstenhagen, D.A.; Kubick, S.
Journal Article
2013Membrane protein synthesis in giant vesicles
Fenz, S.F.; Sachse, R.; Kubick, S.; Schmidt, T.
Conference Paper, Journal Article
2013Synthesis of membrane proteins in eukaryotic cell-free systems
Sachse, R.; Wüstenhagen, D.; Samalikova, M.; Gerrits, M.; Bier, F.F.; Kubick, S.
Journal Article
2012Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system
Stech, M.; Merk, H.; Schenk, J.A.; Stöcklein, W.; Wüstenhagen, D.; Micheel, B.; Duschl, C.; Bier, F.F.; Kubick, S.
Journal Article
2012The protease-activated receptor 1 possesses a functional and cleavable signal peptide which is necessary for receptor expression
Zampatis, D.E.; Rutz, C.; Furkert, J.; Schmidt, A.; Wüstenhagen, D.; Kubick, S.; Tsopanoglou, N.E.; Schülein, R.
Journal Article
2012Toxine aufspüren. Zellfreie Proteinsynthese - Möglichkeiten der Darstellung zytotoxischer Proteine
Kubick, S.
Journal Article
2011Cell-free synthesis and characterization of a novel cytotoxic pierisin-like protein from the cabbage butterfly Pieris rapae
Orth, J.H.C.; Schorch, B.; Boundy, S.; Ffrench-Constant, R.; Kubick, S.; Aktories, K.
Journal Article
2010Protein incorporation in giant lipid vesicles under physiological conditions
Shaklee, P.M.; Semrau, S.; Malkus, M.; Kubick, S.; Dogterom, M.; Schmidt, T.
Journal Article
2010Von der Sequenz bis zur Funktion: Zellfreie Synthese von Membranproteinen auf Biochips
Kubick, S.; Pockrand, M.; Richter, L.; Sachse, R.; Wüstenhagen, D.; Duschl, C.; Bier, F.F.
Conference Paper