Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.
2019A Combined Ultrafiltration/Diafiltration Step Facilitates the Purification of Cyanovirin-N From Transgenic Tobacco Extracts
Opdensteinen, P.; Clodt, J.I.; Müschen, C.R.; Filiz, V.; Buyel, J.F.
Journal Article
2019Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin
Gengenbach, B.B.; Keil, L.L.; Opdensteinen, P.; Müschen, C.R.; Melmer, G.; Lentzen, H.; Bührmann, J.; Buyel, J.F.
Journal Article
2019The Correlation Between DsRed mRNA Levels and Transient DsRed Protein Expression in Plants Depends on Leaf Age and the 5′ Untranslated Region
Jansing, J.; Buyel, J.F.
Journal Article
2019Hitze stabilisiert Malariaimpfstoffkandidaten in komplexen Proteinextrakten
Buyel, J.F.
Journal Article
2019Non-invasive imaging and modeling of liver regeneration after partial hepatectomy
Zafarnia, S.; Mrugalla, A.; Rix, A.; Doleschel, D.; Gremse, F.; Wolf, S.D.; Buyel, J.F.; Albrecht, U.; Bode, J.G.; Kiessling, F.; Lederle, W.
Journal Article
2019Plant Molecular Farming - Integration and Exploitation of Side Streams to Achieve Sustainable Biomanufacturing
Buyel, J.F.
Journal Article
2019Ready-to-Use Stocks of Agrobacterium tumefaciens can Simplify Process Development for the Production of Recombinant Proteins by Transient Expression in Plants
Spiegel, H.; Boes, A.; Perales Morales, C.; Rademacher, T.; Buyel, J.F.
Journal Article
2018Bioreactor-based production of glycoproteins in plant cell suspension cultures
Holland, T.; Buyel, J.F.
Book Article
2018Current status and perspectives of the molecular farming landscape
Spiegel, H.; Stöger, E.; Twyman, R.M.; Buyel, J.F.
Book Article
2018Downstream processing of a plant-derived malaria transmission-blocking vaccine candidate
Menzel, S.; Holland, T.; Boes, A.; Spiegel, H.; Fischer, R.; Buyel, J.F.
Journal Article
2018Expression and purification of human phosphatase and actin regulator 1 (PHACTR1) in plant-based systems
Gengenbach, B.B.; Müschen, C.R.; Buyel, J.F.
Journal Article
2018A linear epitope coupled to DsRed provides an affinity ligand for the capture of monoclonal antibodies
Rühl, C.; Knödler, M.; Opdensteinen, P.; Buyel, J.F.
Journal Article
2018Plants as sources of natural and recombinant anti-cancer agents
Buyel, J.F.
Journal Article
2017Animal component-free Agrobacterium tumefaciens cultivation media for better GMP-compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana
Houdelet, M.; Galinski, A.; Holland, T.; Wenzel, K.; Schillberg, S.; Buyel, J.F.
Journal Article
2017Blanchieren vereinfacht die Reinigung rekombinanter Proteine aus Pflanzen
Buyel, J.F.
Journal Article
2017How Plants Can Contribute to the Supply of Anticancer Compounds
Buyel, J.F.
Book Article
2017Improvement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris
Kastilan, R.; Boes, A.; Spiegel, H.; Voepel, N.; Chudobova, I.; Hellwig, S.; Buyel, J.F.; Reimann, A.; Fischer, R.
Journal Article
2017Production of Functional Anti-Ebola Antibodies in Pichia pastoris
Purcell, O.; Opdensteinen, P.; Chen, W.; Lowenhaupt, K.; Brown, A.; Hermann, M.; Cao, J.; Tenhaef, N.; Kallweit, E.; Kastilan, R.; Sinskey, A.J.; Perez-Pinera, P.; Buyel, J.F.; Lu, T.K.
Journal Article
2017A rapid laser probing method facilitates the non-invasive and contact-free determination of leaf thermal properties
Buyel, J.F.; Gruchow, H.M.; Wehner, M.
Journal Article
2017Very-large-scale production of antibodies in plants: The biologization of manufacturing
Buyel, J.F.; Twyman, R.M.; Fischer, R.
Journal Article
2016Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail
Boes, A.; Spiegel, H.; Kastilan, R.; Bethke, S.; Voepel, N.; Chudobova, I.; Bolscher, J.M.; Dechering, K.J.; Fendel, R.; Buyel, J.F.; Reimann, A.; Schillberg, S.; Fischer, R.
Journal Article
2016Comparison of tobacco host cell protein removal methods by blanching intact plants or by heat treatment of extracts
Buyel, J.F.; Hubbuch, J.; Fischer, R.
Journal Article
2016Determination of the thermal properties of leaves by non-invasive contact-free laser probing
Buyel, J.F.; Gruchow, H.M.; Tödter, N.; Wehner, M.
Journal Article
2016Numeric simulation can be used to predict heat transfer during the blanching of leaves and intact plants
Buyel, J.F.
Journal Article
2016Optimized blanching reduces the host cell protein content and substantially enhances the recovery and stability of two plant-derived malaria vaccine candidates
Menzel, S.; Holland, T.; Boes, A.; Spiegel, H.; Bolzenius, J.; Fischer, R.; Buyel, J.F.
Journal Article
2016Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins
Arfi, Z.A.; Hellwig, S.; Drossard, J.; Fischer, R.; Buyel, J.F.
Journal Article
2016Procedure to evaluate the efficiency of flocculants for the removal of dispersed particles from plant extracts
Buyel, J.F.
Journal Article
2015Cellulose-based filter aids increase the capacity of depth filters during the downstream processing of plant-derived biopharmaceutical proteins
Buyel, J.F.; Opdensteinen, P.; Fischer, R.
Journal Article
2015Controlling the interplay between Agrobacterium tumefaciens and plants during the transient expression of proteins
Buyel, J.F.
Journal Article
2015Depth filters containing diatomite achieve more efficient particle retention than filters solely containing cellulose fibers
Buyel, J.F.; Gruchow, H.M.; Fischer, R.
Journal Article
2015Extraction and downstream processing of plant-derived recombinant proteins
Buyel, J.F.; Twyman, R.M.; Fischer, R.
Journal Article
2015The impact of Pseudomonas syringae type III effectors on transient protein expression in tobacco
Buyel, J.F.; Buyel, J.J.; Haase, C.; Fischer, R.
Journal Article
2015A juice extractor can simplify the downstream processing of plant-derived biopharmaceutical proteins compared to blade-based homogenizers
Buyel, J.F.; Fischer, R.
Journal Article
2015Process development strategies in plant molecular farming
Buyel, J.F.
Journal Article
2015Synthetic polymers are more effective than natural flocculants for the clarification of tobacco leaf extracts
Buyel, J.F.; Fischer, R.
Journal Article
2014Characterization of complex systems using the design of experiments approach
Buyel, J.F.; Fischer, R.
Journal Article
2014Downstream processing of biopharmaceutical proteins produced in plants: The pros and cons of flocculants
Buyel, J.F.; Fischer, R.
Journal Article
2014Flocculation increases the efficacy of depth filtration during the downstream processing of recombinant pharmaceutical proteins produced in tobacco
Buyel, J.F.; Fischer, R.
Journal Article
2014Generic chromatography-based purification strategies accelerate the development of downstream processes for biopharmaceutical proteins produced in plants
Buyel, J.F.; Fischer, R.
Journal Article
2014Molecular Farming in Plants. The Long Road to the Market
Fischer, R.; Buyel, J.F.; Schillberg, S.; Twyman, R.M.
Book Article
2014Molecular Farming: Innovative Produktion pharmazeutischer Proteine in Pflanzen
Buyel, J.F.; Reimann, A.; Drossard, J.; Fischer, R.
Journal Article
2014Rational design of a host cell protein heat precipitation step simplifies the subsequent purification of recombinant proteins from tobacco
Buyel, J.F.; Gruchow, H.M.; Boes, A.; Fischer, R.
Journal Article
2014Scale-down models to optimize a filter train for the downstream purification of recombinant pharmaceutical proteins produced in tobacco leaves
Buyel, J.F.; Fischer, R.
Journal Article
2013Commercial aspects of pharmaceutical protein production in plants
Fischer, R.; Schillberg, S.; Buyel, J.F.; Twyman, R.M.
Journal Article
2013Predictive models for the accumulation of a fluorescent marker protein in tobacco leaves according to the promoter/5' UTR combination
Buyel, J.F.; Kaever, T.; Buyel, J.J.; Fischer, Rainer
Journal Article
2013Processing heterogeneous biomass: Overcoming the hurdles in model building
Buyel, J.F.; Fischer, R.
Journal Article
2013The use of quantitative structure-activity relationship models to develop optimized processes for the removal of tobacco host cell proteins during biopharmaceutical production
Buyel, J.F.; Woo, J.A.; Cramer, S.M.; Fischer, R.
Journal Article
2012Extraction, purification and characterization of the plant-produced HPV16 subunit vaccine candidate E7 GGG
Buyel, J.F.; Bautista, J.A.; Fischer, R.; Yusibov, V.M.
Journal Article
2012Predictive models for transient protein expression in tobacco (Nicotiana tabacum L.) can optimize process time, yield, and downstream costs
Buyel, J.F.; Fischer, R.
Journal Article